Figure 1. Templates from diff erent sources were amplified by TIANGEN Taq MasterMix II and the common Taq Mix from Supplier TR respectively to detect the stress resistance of the reagents. The results show that TIANGEN products can amplify the target fragments from crude genomic templates and bacterial culture, and the stress resistance is better than that of Supplier TR. A: Crude genomic template extracted by TIANGEN TIANcombi DNA Lyse&Det PCR Kit. Prp/DN: Crude extraction and detection of human blood samples. Rice: Crude extraction and detection of rice samples. B: Colony PCR. The PCR fragment is 700 bp. M: TIANGEN Marker III |
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Good universality for templates from different sources and with different lengths Figure 2. Fragments of different sources and lengths were amplified using TIANGEN Taq MasterMix II (A) and ordinary Taq Mix of Supplier TK (B), Supplier TR (C), Supplier V (D) and Supplier G (E) respectively. The results show that the comprehensive performance of TIANGEN products is the best in terms of amplification capability, specificity and universality.M: TIANGEN Marker III1: Soybean genomic DNA template (120 bp); 2-3: Rice genomic DNA template (694 bp, 2258 bp); 4: Cotton genomic DNA template (200 bp); 5: Escherichia coli genomic DNA template (2298 bp); 6-7: Mouse genome DNA template (1 kb, 2 kb); 8-10: Rat genomic DNA template (1 kb, 2 kb, 2080 bp); 11-18: Human genome DNA template (300 bp, 448 bp (GC%: 74.8%), 1100 bp, 750 bp, 1000 bp, 1090 bp (GC%: 70.4%), 2 kb, 4 kb) |
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High sensitivity Figure 3. Different concentrations of rat and human DNA fragments were amplified using TIANGEN Taq MasterMix II (A), ordinary Taq Mix of Supplier V (B) and Supplier TK (C), respectively, to detect the amplification sensitivity. The results show that TIANGEN product could amplify the target fragment from the genome template as low as 0.01 ng, and its sensitivity is better than that of the products from Supplier V and TK.M: TIANGEN Marker III, N: NTCTemplate input 1-8: 200 ng, 100 ng, 50 ng, 20 ng, 10 ng, 1 ng, 0.1 ng, 0.01 ng. |