Best Price on Ujian Rt Pcr - FastKing RT Kit (With gDNase) – TIANGEN


Product Detail

Product Tags

Related Video

Feedback (2)

Our development depends on the advanced equipment, excellent talents and continuously strengthened technology forces for Trizol Dna, Dna Extraction Protocols, Nucleotide And Nucleic Acid, If you are looking for a good quality, fast delivery, best after service and good price supplier in China for long-term business relationship, we will be your best choice.
Best Price on Ujian Rt Pcr - FastKing RT Kit (With gDNase) – TIANGEN Detail:

Features

■ High efficiency: The FastKing RT Enzyme is modified with hydrophobic motif, with RT efficiency more than 95%.
■ Sensitive: As low as 1 ng templates can be accurately identified.
■ Resistance: Capable of reverse transcription of complex templates, with perfect resistance to impurities.
■ Flexible: Genomic DNA  removal and reverse transcription was completed separately. Primers were mixed separately in a tube, flexibly to change other primers.

Specification

Type: Gene modified reverse transcriptase, gDNase
Procedures: Two-step (genomic DNA removal and RT)
RT efficiency: >95%
Template: 1 ng- 2 μg
Operation time: ~21 min
Applications: The reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction.

21 min reaction in one-tube

It only takes 21 min to complete the gDNA removal and efficient reverse transcription process in the same tube without replacing the reaction tube and independent DNase I treatment process. Compared with the traditional method that requires 12-step operation and 140 min reaction, it greatly simplifies the operation steps and saves a lot of operation time.

21 min reaction in one-tube

Outstanding quality of King RTase

——Ultra-high reverse transcription efficiency
——Reverse transcription efficiency is over 95%
The general reverse transcriptase has a reverse transcription efficiency of 40-60%, and cDNA yield can be increased by a higher RNA loading amount. King reverse transcriptase can achieve a reverse transcription efficiency of more than 95% due to its unique high affinity for RNA templates. Therefore, subsequent experiments can be satisfied without the need of a large amount of RNA input, which saves RNA and enables high purity and high yield of cDNA.
Outstanding quality of King RTase

Easily read through complex templates

——Easily read through high GC and complex templates
Single-stranded RNA has a wide range of complex secondary structure regions due to hydrogen bonding between strands. Ordinary reverse transcriptase may lead to termination of reverse transcription when encountering complex secondary structure, thus unable to successfully complete cDNA synthesis. However, the new generation of King reverse transcriptase has a unique structural domain, which can destroy the hydrogen bond between RNA strands, thus opening the complex secondary structure of RNA and ensuring the smooth reverse transcription.

Easily read through complex templates


Product detail pictures:

Best Price on Ujian Rt Pcr - FastKing RT Kit (With gDNase) – TIANGEN detail pictures


Related Product Guide:

It is a good way to enhance our products and solutions and repair. Our mission will be to build creative solutions to consumers with a great experience for Best Price on Ujian Rt Pcr - FastKing RT Kit (With gDNase) – TIANGEN , The product will supply to all over the world, such as: Birmingham, moldova, Cape Town, In order to carry out our goal of "customer first and mutual benefit" in the cooperation, we establish a professional engineering team and a sales team to supply the best service to satisfy our customers' requirements. Welcome you to cooperate with us and join us. We are your best choice.
product_certificate04 product_certificate01 product_certificate03 product_certificate02
×
  • Sales manager is very enthusiastic and professional, gave us a great concessions and product quality is very good,thank you very much!
    5 Stars By Isabel from Guinea - 2017.11.11 11:41
    The factory can meet continuously developing economic and market needs, so that their products are widely recognized and trusted, and that's why we chose this company.
    5 Stars By Erica from Luxemburg - 2017.09.26 12:12
    Write your message here and send it to us