China Factory for Rt Pcr Amplification - Multi PCR Kit – TIANGEN

Features

■ High specificity: Chemically modified hot-start enzyme with activation time up to 15 min to ensure high specificity amplification.
■ High sensitivity: Low copy amplification and high efficiency amplification of multiplex PCR.
■ Simple operation: The enzyme is inactive at low temperature and room temperature, and the reagent can be prepared at room temperature.

Activity Definition

1 unit (U) HotStart Taq DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74℃ within 30 min using activated salmon sperm DNA as template/primer.

Main Technical Parameters

It has 5′-3′ exonuclease activity and no 3′-5′ exonuclease activity with the strongest specificity. The 3′ end of PCR product is A, which can be directly used for TA cloning.

Specification

Type: Chemical-modified HotStart DNA polymerase
Applications: Multiplex PCR experiment, high specificity detection experiment, amplification of low-copy gene, PCR amplification of templates with complex structures (such as genomic DNA, cDNA, etc.).

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