EndoFree Maxi Plasmid Kit

Features

■ Fast and high yield: 200 μg-1.5 mg plasmid DNA yield in 1 hour with high proportion of super-coiled structure.
■ High-purity: High-purity plasmid DNA are acquired by unique buffer system and Spin Column CP6.
■ Excellent transfection efficiency: Suitable for transfection experiments of most cell lines.
■ Wide range of applications: Suitable for restriction enzyme digestion, transformation, sequencing, microinjection, gene silencing and transfection experiments.

Plasmid Yield

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

High/low copy plasmid extraction Low-copy plasmid pBR322, extracted from 200 ml bacterial culture using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 0.6 μg/μl. High-copy plasmid pBS, extracted from 100 ml bacterial culture using EndoFree Maxi Plasmid Kit ,was eluted in 1 ml Buffer TB, with a concentration of 1.2 μg/μl. MIV: TIANGEN DNA Marker IV pBR322: 2 μl, pBS : 2 μl

pEGFP obtained with EndoFree Maxi Plasmid Kit were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells by TIANGEN TIANfect. Expression of GFP were detected in 24 hours after transfection.

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