EndoFree Midi Plasmid Kit

For rapid purification of medium amount of endotoxin-free transfection grade plasmid DNA.

The EndoFree Midi Plasmid Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. The special Buffer EBT and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour, which is convenient and quick.

Cat. No	Packing Size
4992853	10 preps

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Product Detail

Product Tags

Features

■ Suitable for extracting plasmids from 50-100 ml bacteria culture medium.
■ The purified plasmid contain very low endotoxin and can meet the requirements of high quality transfection.
■ Easy to operate with a unique and rapid endotoxin removal method.

Applications

The purified plasmid DNA can be applied in molecular biology experiments and cell biology experiments such as restriction endonuclease digestion, sequencing, transformation, transfection, microinjection, etc.

Plasmid Yield

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Workflow

Experimental Example

	Plasmid pEGFP-C1 is purified by the EndoFree Midi Plasmid Kit from 50 ml overnight cultured bacteria medium. The plasmid concentration was measured by Nanodrop and the yield was calculated. The result shows that the plasmid yield by EndoFree Mini Plasmid Kit is higher than that by relevant product from Supplier A.
	The plasmid pEGFP-C1 extracted by EndoFree Midi Plasmid Kit is transfected into 80% confluence 293T cells by TIANGEN TIANfect. Expression of GFP was detected 24-36 h after transfection. The result shows that the transfection efficiency was 80%-90%.