Factory wholesale Rt Pcr Qualitative - 2×Taq PCR Mix – TIANGEN

Activity Definition

The activity of 1 unit (U) Taq DNA Polymerase is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74℃ within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

The enzyme has 5′-3′ polymerase activity and 5′-3′ exonuclease activity, and without 3′-5′ exonuclease activity. The extension speed of DNA polymerization is 1-2 kb/min at 70-75℃ . The PCR product has 3′-dA overhangs, which can be directly cloned in TA-cloning vector.

Applications

It is generally used for amplification, primer extension, sequencing, and A-tailing at the blunt end of DNA that’s <6 kb and has low fidelity requirements.

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