FastKing One Step RT-qPCR Kit (Probe)

Features

■ No cross-contamination: Reverse transcription and fluorescence quantification are completed in one step to avoid cross contamination.
■ Sensitive: Templates as low as 1 ng can be accurately identified, especially suitable for templates with low abundance.
■ Stress resistance: Can be applied on complex templates, perfect against foreign interference, suitable for various templates
■ Simple operation: Optimize product composition and improve experimental efficiency.

Specification

Type: Gene modified reverse transcriptase, antibody-modified hot-start DNA polymerase, probe
Template: 10 pg-1 μg
Applications: The kit is suitable for probe one-step Real-Time RT-PCR, it can accurately and simply perform mRNA expression analysis. It is especially suitable for detection of trace RNA on various real time PCR instruments

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Total RNA of human 293T cells, corn leaves and enteroviruses were extracted respectively and the housekeeping genes were reverse transcribed and quantified by FastKing One Step RT-qPCR Kit (Probe) and relevant product from Supplier A respectively in one step. The results show that TIANGEN FastKing One Step RT-qPCR Kit (Probe) has high fluorescence value, lower CT value, clear gradient and good repeatability, which obviously outperforms the product from Supplier A. A: 293T cells. Orange line:TIANGEN, Red line: Supplier A. B: Corn leaf. Blue line: TIANGEN,Green line: Supplier A. C: Enterovirus. Pink line: TIANGEN,Purple line: Supplier A.

8, 40, 400 copies of In vitro transcribed SARS-CoV2 were detected by TIANGEN FastKing One Step RT-qPCR Kit (Probe) (Red) and relevant product from Supplier A (Blue). The result shows that TIANGEN FastKing One Step RT-qPCR Kit (Probe) can detect SARS-CoV-2 effectively with high sensitivity, as low as 8 copies of the IVT RNA can be detected for both ORF1a and N signal.

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