Genomic DNA was extracted from 100 μl of human blood sample using TIANamp Blood DNA Kit. 50 ng DNA was loaded for the real time PCR detection. According to NCBI SNP library, the known SNP of FEN1 gene (RS 174538) is selected and detected using Roche LightCycle480. The results are as follows:
The results show that HRM Analysis Kit is an ideal SNP locus analysis method with high repeatability of melting curve of the same genotype, clear resolution of different genotypes and no misjudgment.
SNP information: ……CGGAAGAACACGTCG[A/G]CAGGAGCAGGCGCCT…… (Allele Frequency: A=0.314, G=0.686)
Primer: For 108 bp fragment (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)
