■ High conversion efficiency: The optimized reaction system ensures the stability of the product and high efficiency of double strand cDNA synthesis.
■ Simple operation: Integrated reaction process and simplified operation steps.
■ Good compatibility: The purified double stranded cDNA of the reaction product can be directly used for the construction of RNA-Seq library.
Type: RNA fragmentation and cDNA synthesis in the preparation of non-directional RNA library
Sample: Total RNA
Target: mRNA
Starting sample input: Total RNA samples is 10 ng-1 μg, and that of mRNA sampe is as low as 500 pg
Operation time: 2.5 hour
Downstream applications: End repair and 3’ end dA-tailing of double-stranded cDNA
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