Professional Design Real Time Pcr Primer - 2×Taq PCR Mix – TIANGEN

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We have our own product sales staff, style crew, technical group, QC staff and package staff. We now have strict high quality management procedures for each approach. Also, all of our workers are experienced in printing subject for Spooling Of Dna, Nucleic Membrane, Glycerol Nucleic Acid, We believe that in good quality more than quantity. Before export of the hair there is strict top quality control check during treatment as per international good quality standards.

Professional Design Real Time Pcr Primer - 2×Taq PCR Mix – TIANGEN Detail:

Activity Definition

The activity of 1 unit (U) Taq DNA Polymerase is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74℃ within 30 min using activated salmon sperm DNA as template/primer.

Quality Control

The purity by SDS-PAGE detection is more than 99%; No activity of exogenous nuclease is detected; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Main Technical Parameters

The enzyme has 5′-3′ polymerase activity and 5′-3′ exonuclease activity, and without 3′-5′ exonuclease activity. The extension speed of DNA polymerization is 1-2 kb/min at 70-75℃ . The PCR product has 3′-dA overhangs, which can be directly cloned in TA-cloning vector.

Applications

It is generally used for amplification, primer extension, sequencing, and A-tailing at the blunt end of DNA that’s <6 kb and has low fidelity requirements.

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