RNA Easy Fast Plant Tissue Kit

For purification of high-quality total RNA from plant tissues.

The RNA Easy Fast Plant Tissue Kit is a rapid RNA extraction kit for plant tissues developed based on the genomic DNA removal technology exclusively developed by TIANGEN. Toxic reagents such as β-mercaptoethanol or DTT are not needed during the operation, and the whole process of RNA extraction can be completed within 30 minutes. It is not only suitable for leaf samples of common plants such as wheat and corn, but also for polysaccharide/polyphenol-rich samples such as Malus spectabilis leaf, cotton leaf, chrysanthemum leaf, hibiscus flower, potato tuber, watermelon, cucumber, pine needle, etc.

Cat. No Packing Size
4992880 50 preps

Product Detail

Product Tags

Features

■ Simple and fast: Total RNA extraction from plant samples can be completed within 30 minutes.
■ Strong compatibility: This kit is not only suitable for common stem and leaf samples, but also for polysaccharide/polyphenol-rich samples.
■ Safe and low toxic: Toxic reagents such as β-mercaptoethanol, DTT, chloroform, phenol are not needed.

Applications

■ Suitable for a downstream operation, including RT-PCR, RT-qPCR, chip hybridization, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning, etc.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Experimental Example The total RNA of 65 mg leaf samples (clove leaves, hibiscus leaves, wheat leaves, rice leaves, ugli fruit leaves, Prunus cerasifera leaves, fig leaves, daylily leaves, Kerria japonica leaves), 150 mg fungus samples (Lentinus edodes) and 200 mg petal samples (day-lily petals) were extracted using RNA Easy Fast Plant Tissue Kit.Agilent Bioanalyzer 2100 analysis result of total RNA from Day-lily petals.
Experimental Example Agilent Bioanalyzer 2100 analysis result of total RNA from Day-lily petals.
 Experimental Example RNA extracted from various samples listed in the table using RNA Easy Fast Plant Tissue Kit.
Elution volume: 50 μl; Loading volume: 2 μl.
The electrophoresis was conducted at 6 V/cm for 15 min on a 1% agarose.

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1  Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2  Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1  The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2  Sample amount is too large

---- Reduce sample amount.

A-3  RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4  Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5  Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1  Sample amount is too large

---- Reduce sample amount.

A-2  Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).


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