RNA Easy Fast Tissue/Cell Kit

For purification of high-quality total RNA from animal tissues/cells.

RNA Easy Fast Tissue/Cell Kit is a rapid RNA extraction kit for animal tissues/cells samples. It is developed based on the genomic DNA removal technology exclusively developed by TIANGEN. A large number of different samples can be processed at the same time with the fast procedure within 30 minutes. RNA isolated by this product can directly serve as templates for downstream detection or other applications.

Cat. No Packing Size
4992732 50 preps

Product Detail

Product Tags

Features

■ Fast operation: RNA could be obtained within 30 min.
■ High purity: Silica membrane gets rid of most impurities and gDNA removal column gets rid of genomic DNA.
■ Wide use: Suitable for different samples such as tissue, cell and bacteria.

Specification

Type: Spin column based
Sample and starting volume:  10-20 mg tissue or <107 cells
Target: RNA
Operation time: ~30 min
Downstream applications: RT-PCR/RT-qPCR, Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection assay, molecular cloning, etc.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Experimental Example Experimental Example RNA extracted from 15 mg rat liver sample using the RNA Easy Fast Tissue/Cell Kit and relevant product from supplier A and T.
Elution volume: 100 μl; Loading volume: 3 μl
M: TIANGEN Marker D15000
Experiment result: RNA Easy Fast Tissue/Cell Kit has higher extraction rate than the relevant product from supplier A and T.

 

 

 

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1  Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2  Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1  The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2  Sample amount is too large

---- Reduce sample amount.

A-3  RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4  Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5  Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1  Sample amount is too large

---- Reduce sample amount.

A-2  Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).


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