RNAprep Pure Micro Kit

For purification of high-quality total RNA from micro amount of tissues or cells.

The RNAprep Pure Micro Kit uses a highly efficient, nucleic acid-specific centrifugal adsorption column and a unique buffer system to rapidly extract total RNA from a wide range of different types of microsamples. This kit includes Carrier RNA, which can easily capture trace nucleic acids from the system. The extraction of RNA by this kit is convenient, rapid and reproducible with high yield. The reaction can be completed within 30-40 minutes. The kit can selectively remove all RNA that is <200 nt (5.8S rRNA, 5S rRNA and tRNAs, etc.), while enrich, isolate and purify all RNA that is >200 nt. The extracted total RNA is extremely pure and free of DNA and protein contamination.

Cat. No	Packing Size
4992859	50 preps

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Product Detail

Product Tags

Features

■ Capable of purifying high-quality RNA from trace amount samples, such as micro-dissected tissue, fibrous tissue and cells.
■ Unique DNase I minimizes genomic DNA contamination.
■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.
■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

Applications

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ Real-Time PCR.
■ Chip analysis.
■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Total RNA of 1×10⁶, 1×10⁵, 1×10⁴, 1×10³, 1×10², 10 Hela cells were extracted using RNAprep Pure Micro Kit. RT-qPCR was performed using Quant qRT-PCR (SYBR Green) Kit of TIANGEN.

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1 Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1 The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2 Sample amount is too large

---- Reduce sample amount.

A-3 RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4 Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5 Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1 Sample amount is too large

---- Reduce sample amount.

A-2 Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).

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