RNAprep Pure Tissue Kit

For purification of up to 100 μg total RNA from animal tissues.

The RNAprep Pure Tissue Kit provides a fast, simple, and cost-effective method for purification of total RNA from animal tissues by using effective spin column and unique buffer system. The kit includes RNase-Free spin columns CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 40-50 minutes with high-purity and is free of protein and genomic DNA contamination.

Cat. No	Packing Size
4992236	50 preps

Send email to us

Product Detail

Product Tags

Features

■ Optimized buffers for animal tissues make the process simple and convenient.
■ Unique DNase I minimizes genomic DNA contamination.
■ The higher-purity and ready-to-use RNA is suitable for sensitive downstream applications.
■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, and no CsCl gradients centrifugation are needed, which makes the process safe and reliable.

Applications

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ Real-Time PCR.
■ Chip analysis.
■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Material: 20 mg embryo (13 days), 15 mg kidney, 10 mg liver, 15 mg spleen, 10 mg thymus, 20 mg lung
Method: Total RNA from different tissue samples of rat was purified using the RNAprep Pure Tissue Kit.
Results: The agarose gel electrophoresis picture is showed above. 2-4 μl of 100 μl eluates were loaded per lane.
M: TIANGEN DNA Marker III;
Lane 1-2: Embryo (13 days); Lane 3: Kidney; Lane 4-6: Liver; Lane 7: Spleen; Lane 8: Thymus; Lane 9: Lung.
The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose gel..

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1 Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1 The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2 Sample amount is too large

---- Reduce sample amount.

A-3 RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4 Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5 Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1 Sample amount is too large

---- Reduce sample amount.

A-2 Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).

Previous: RNAprep Pure Cell/Bacteria Kit

Next: RNAprep Pure FFPE Kit