RNAsimple Total RNA Kit

For the high-efficient total RNA extraction using the widely used centrifugal column.

The RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.
This kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5×10⁶ cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.

Cat. No	Packing Size
4992858	50 preps

Send email to us

Product Detail

Product Tags

Features

■ The high-purity ready-to-use RNA is suitable for sensitive downstream applications.
■ Wide applications: The purified RNA can be applied to various experimental samples.
■ The experiment can be completed in 1 hour with simple operations.

Applications

■ RT-PCR
■ Northern Blot, Dot Blot.
■ Real-Time PCR
■ PolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Workflow

Experimental Example

Material: 20 mg rat spleen tissue
Method: The total RNA of rat spleen tissue was isolated using the RNAsimple Total RNA Kit.
Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1 Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1 The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2 Sample amount is too large

---- Reduce sample amount.

A-3 RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4 Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5 Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1 Sample amount is too large

---- Reduce sample amount.

A-2 Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).

Previous: RNAclean Kit

Next: RNAprep Pure Micro Kit