Talent qPCR PreMix (SYBR Green)

Good resistance to impurity interference and rapid quantification of complex templates.

This product is a special reagent for Real-Time PCR using SYBR Green I chimeric fluorescence. The 2×Talent qPCR PreMix adopts antibody-modified Anti Taq DNA polymerase, and the unique qPCR Buffer system can ensure the product to carry out highly sensitive and fast qPCR reaction on all Real-Time PCR instruments. The qPCR reaction time can be shortened by 50%. In addition, the addition of H-Competitor factor and EP component in Buffer makes the product have a wide range of sample universality. It has very good applicability to templates with complex advanced structures, templates with more PCR inhibitor residues, long fragment amplification, etc.
The product also has the characteristics of high amplification rate, high amplification specificity, wide credible range, etc., and can obtain results faster without affecting the PCR effect.

Cat. No Packing Size
4992882 20 µl×125 rxn
4992775 20 µl×500 rxn
4992866 20 µl×5000 rxn

Product Detail

Product Tags

Features

■ Highly sensitive antibody modified polymerase can shorten the reaction time by half.
■ The H-Competitor competes with hydrogen bond, which ensures higher amplification efficiency of templates with high GC content, complex secondary structure and long templates.
■ EP component stabilizes PCR system, effectively protects enzyme activity and resists interference of various inhibitors.
■ Colorless transparent tube replaces brown tube, so that the remaining amount is visible.

Applications

It is suitable for expression analysis, nucleic acid detection and other types of experiments on various real time PCR instruments by SYBR Green method, especially for high GC, complex secondary structure, high impurity residue and long template quantification.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Experimental Example

Human UH11 gene (GC: 73.4%) was quantified by TIANGEN Talent qPCR PreMix (left) and relevant product from Supplier A (middle) and B (right). The results show that compared with other suppliers, Talent qPCR PreMix has clear quantitative curves for 5 concentration gradients, lower Ct value, higher fluorescence value, and single melting curve peak, indicating Talent qPCR PreMix has good adaptability and amplification efficiency to high GC templates.


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