Figure 1: qPCR standard curve results show good repeatability cDNA samples of 7 μl NIH 3T3 cells are diluted 4 times with 21 μl of water at a ratio of 1: 4. Roche LightCycler 480 real-time fluorescence quantitative PCR instrument and SuperReal PreMix Plus(SYBR Green) were used for the detection. |
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Figure 2: Higher precision compared to manual pipetting (Left: Manual; Right: TEasy Automated Pipetting) 4 repeats of human GAPDH amplification (top curve). Add 2 μl cDNA to 18 μl MasterMix to form 20 μl reaction system. Roche LightCycler 480 real-time fluorescence quantitative PCR instrument and SuperReal PreMix Plus (SYBR Green) were used for the detection. |