TGuide FFPE DNA One-Step Kit

One-step extraction of genome DNA from FFPE samples.

TGuide FFPE DNA One-Step Kit is specially designed to extract genome DNA from paraffin tissue samples with TGuide automated nucleic acid extractors. This kit adopts a one-step heating method, so that deparaffinization and tissue lysis can be simultaneously carried out, and harmful reagents such as xylene are not contained. This kit has two extraction options: For small amount of samples, choose 2 hours lysis; For large amount of samples, choose 16 hours overnight lysis. The kit adopts cellulose embedded magnetic beads technology to efficiently extract genome DNA.

Cat. No	Packing Size
OSR-M405	48 preps

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Applications

Purified DNA can be directly used in various conventional operations, including enzymatic digestion, PCR, library construction, Southern blot and other experiments.

Features

■ In-machine deparaffinization: The deparaffinization and extraction steps can be completed automatically by simply putting untreated paraffin embedded samples into reagent cartridge and then adding Sula Oil and Proteinase K.
■ Reliable results: The obtained genome DNA is free from RNA and protein contamination, and can be directly used for PCR or fluorescence quantitative PCR.
■ Safe and harmless: Organic solvents that are harmful to human body such as phenol chloroform are not needed.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

The results showed that for large volume samples, the extraction yields of the two methods were comparable; for samples with medium and small volume, the yield of the one-step deparaffinization method (perform with TGuide) was greater than that of the conventional deparaffinization method. Therefore, the one-step deparaffinization method not only simplifies the operation, reduces the risk, but also improves the extraction efficiency.
Note:
Large volume sample: the sectioning area is within 2.5-4 cm² and the thickness is within 5-20 μm.
Medium volume sample: the sectioning area is within 1.5-2.5 cm² and the thickness is within 5-20 μm.
Small volume sample: the sectioning area is within 0.2-1.5 cm² and the thickness is within 5-20 μm.

FAQ

Q: Little or no DNA in the eluent.

A-1 Low concentration of cells or virus in the starting sample —Enrich the concentration of cells or viruses.

A-2 Insufficient lysis of the samples —The samples has not been mixed thoroughly with the lysis buffer. It is suggested to thoroughly mix by pulse-vortexing for 1-2 times. —Insufficient cell lysis caused by the activity decrease of proteinase K. —Insufficient cell lysis or protein degradation due to insufficient warm bath time. It is suggested to cut the tissue into small pieces and extend the bath time to remove all the residue in the lysate.

A-3 Insufficient DNA adsorption. —No ethanol or low-percentage instead of 100% ethanol was added before the lysate was transfer to the spin column.

A-4 The pH value of elution buffer is too low. —Adjust the pH to between 8.0-8.3.

Q: DNA does not perform well in downstream enzymatic reaction experiments.

Residual ethanol in the eluent.

—There is residual washing buffer PW in the eluent. The ethanol can be removed by centrifuging the spin column for 3-5 min, and then placing at room temperature or 50℃ incubator for 1-2 min.

Q: DNA degradation

A-1 The sample is not fresh. —Extract a positive sample DNA as control to determine whether the DNA in the sample has degraded.

A-2 Improper pre-treatment. —Caused by excessive liquid nitrogen grinding, moisture regaining, or too large amount of the sample.

Q: How to perform the pretreatment for gDNA extraction?

The pretreatments should vary for different samples. For plant samples, make sure to thoroughly grind in liquid nitrogen. For animal samples, homogenate or thoroughly grind in liquid nitrogen. For samples with cell walls that are hard to break, such as G+ bacteria and yeast, it is suggested to use lysozyme, lyticase or mechanical methods to break the cell walls.

Q: What is the difference between the three plant gDNA extraction kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit adopts a column-based method that requires chloroform for the extraction. It is especially suitable for various plant samples, as well as plant dry powder. Hi-DNAsecure Plant Kit is also column-based, but with no need of phenol/chloroform extraction, making it safe and non-toxic. It is suitable for plants with high polysaccharides and polyphenol content. 4992709/4992710 DNAquick Plant System adopts a liquid-based method. Phenol/chloroform extraction is not needed as well. The purification procedure is simple and fast with no limit for the sample start amounts, so users can adjust the amount flexibly according to the experimental requirements. Large size of gDNA fragments can be obtained with high yield.

What's the estimated yield of gDNA from 1 ml blood sample by TIANamp Blood DNA Kit?

The genomic DNA was extracted from different volumes of human whole blood samples by TIANamp Blood DNA Kit. The results are as follow. The results are listed as reference only, the actual extraction results depends on the conditions of samples.

Q: Can 4992207/4992208 and 4992722/4992723 be used to extract blood clots DNA?

The blood clot DNA extraction can be performed using the reagents provided in these two kits by simply changing the protocol to the specific instruction for the blood clot DNA extraction. The soft copy of the blood clot DNA extraction protocol can be issued upon requesting.

Q: When applying TIANamp Genomic DNA Kit, how to break the fresh tissues into cell suspension?

Suspend the fresh sample with 1 ml PBS, normal saline or TE buffer. Completely homogenize the sample by a homogenizer and collect the precipitate to the bottom of a tube by centrifuging. Dispose the supernatant, and resuspend the precipitate with 200 μl buffer GA. The following DNA purification can be performed according to the instruction.

Q: How to choose the product for the DNA extraction from plasma, serum and body fluid samples?

For the purification of gDNA in plasma, serum and body fluid samples, TIANamp Micro DNA Kit is recommended. For the purification of virus gDNA from serum/plasma samples, TIANamp Virus DNA/RNA Kit is recommended. For the purification of bacterial gDNA from serum and plasma samples, TIANamp Bacteria DNA Kit is recommended (lysozyme should be included for positive bacterial). For saliva samples, Hi-Swab DNA Kit and TIANamp Bacteria DNA Kit are recommended.

Q: How to choose the kits for the gDNA extraction from fungi samples?

DNAsecure Plant Kit or DNAquick Plant System are recommended for fungal genome extraction. For yeast genome extraction, TIANamp Yeast DNA Kit is recommended (lyticase should be self-prepared).

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