TIANprep N96 Plasmid Kit

Real-time monitoring of lysis status and high-throughput extraction of high-purity plasmids

The membrane of the N96 Plate CP3 in this kit adopts a novel silica matrix material uniquely developed by TIANGEN, which can efficiently and specifically adsorb plasmid DNA, remove protein and other impurities to the greatest extent, thus ensuring the purity of the extracted plasmid. Up to 1.3 ml of high-copy plasmid bacterial culture can be processed per well, from which up to 5-10 μg of plasmid DNA can be rapidly extracted (the yield is related to factors such as plasmid copy number, bacterial state, etc.). Plasmid DNA extracted by the kit can be used in molecular biology experiments such as restriction enzyme digestion, sequencing, library screening, ligation and transformation.

Cat. No	Packing Size
4992892	4 preps
4992893	24 preps

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Product Detail

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Features

■ Fast extraction speed.
■ Equipped with a unique TIANRed reagent, it can clearly identify the degree of lysis of the sample in the extraction process to ensure high efficiency and high purity of plasmid DNA.
■ High purity: The purified plasmid DNA can be directly used in downstream experiments.
■ This kit can be used in high throughput automated nucleic acid extraction workstations.

TIANRed Color indicator

TIANRed Color indicator:

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

	Plasmid DNA was extracted from 1 ml of overnight cultured Escherichia coli (TOP10) broth (LB medium) using TIANprep Highpure N96 Plasmid Kit, and an average of 5-6 μg of high purity pBS plasmid was obtained. The plasmid was loaded into each well of a 1% agarose gel, and was detected by electrophoresis
	Plasmid DNA was extracted from 1 ml of overnight cultured Escherichia coli (TOP10) broth (LB medium) using TIANprep Highpure N96 Plasmid Kit, and an average of 5-6 μg of high purity pBS plasmid was obtained. Vector: pBS; Plasmid strain: TOP10. The plasmid was sequenced by ABI. PRISM 3730XL DNA sequencer.