Reverse transcription ability of TIANScript II RT Kit for different length fragments Method: Reverse transcription: Refer to the instruction manual of TIANScript II RT Kit. Results: The gel picture shows the amplification results of 10 target genes with different lengths after reverse transcription of 1 μg of total RNA. 2 μl of reverse transcription products were loaded per lane. Amplification system (PCR): 20 μl; Sample load: 5 μl; Marker: D15000+1 kb DNA Ladder; Gel concentration: 1%; Electrophoresis conditions: 6 V/cm, 20 min Diagram of each lane: M: DNA Marker; 1: Product length: 120 bp; 2: Product length: 1 kb; 3: Product length: 2.5 kb; 4: Product length: 3.2 kb; 5: Product length: 4.6 kb; 6: Product length: 6.8 kb; 7: Product length: 7.6 kb; 8: Product length: 8.9 kb; 9: Product length: 10 kb; 10: Product length: 12 kb; |
|
Comparison of efficiency and specificity of TIANScript II RT Kit and products from other suppliers in reverse transcription of long templates Materials: Total RNA of human adherent cells. RT-PCR starting amount: 2 μl reverse transcription product (50 ng/μl) Method: Reverse transcription: Refer to to the instruction manual of TIANScript II RT Kit. Results: The gel picture shows the amplification results of 6 target genes with different lengths after reverse transcription of 1 μg total RNA of human adherent cells using M-MLV from Supplier A and TIANGEN TIANScript II RT Kit. Amplification system (PCR): 20 μl; Sample load: 5 μl; Marker: DNA MarkerIII; Gel concentration: 1%;Electrophoresis conditions: 6 V/cm, 20 min. Diagram of each lane: M: DNA Marker; 1: Amplification results of the cDNA reverse transcribed using TIANScriptII RT Kit; 2. Amplification results of the cDNA reverse transcribed using relevant product from Supplier A. The length of gene 1 product is 1.3 kb; The length of gene 2 product is 3.0kb; The length of gene 3 product is 5.0 kb; The length of gene 4 product is 7.5 kb. |