Hot-start to ensure product specificity Figure 1. Ultra HiFi has excellent hot-start function to ensure the specificity of amplification products. Molecular beacons method was applied (Ma et al., Anal Biochem, 2006). |
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Excellent high fidelity, 50 times higher than Taq Polymerase Figure 2. The fidelity of Ultra HiFi is 50 times higher than that of common Taq polymerase. The polymerization fidelity of Taq polymerase (without corrective activity) is used as reference. |
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Rapid amplification and long fragments can be amplified fast Fig. 3. Ultra HiFi can extend up to 5 sec/kb for fragments smaller than 4 kb. For long fragments, the amplification time can be extended appropriately. For fragments more than 15 kb, the extent speed can be up to 30 sec/kb. M: TIANGEN D15000 Marker |
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Strong universality and high specificity, easy to read high GC and long fragments from different sources Figure 4. Ultra HiFi has high specificity to ensure amplification success rate and product quantity for different types of templates. A. Ultra HiFi amplification results B. Hi-Fi enzyme amplification results of Supplier K C. Hi-Fi enzyme amplification results of Supplier N M: TIANGEN D15000 Marker Lane 1-5. Amplification results of templates with different length: 1. 750 bp; 2. 1 kb; 3. 2 kb; 4. 4 kb; 5. 6 kb Lane 6. Amplification result of high GC template: 1915 bp (GC%: 70%); Lane 7-11. Amplification result of 2 kb templates from various genome: 7. Rat; 8. Rice; 9. Wheat; 10. Corn; 11. Bacteria; Lane 12-14. 8 kb long fragment amplification result: 12. Rice; 13. Corn; |